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Literature summary extracted from

  • Petrovan, R.J.; Ruf, W.
    Residue Met(156) contributes to the labile enzyme conformation of coagulation factor VIIa (2001), J. Biol. Chem., 276, 6616-6620.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.21 M156Q mutation has no influence on the amidolytic and proteolytic activity of tissue factor bound enzyme, increased affinity for tissue factor Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.21 1.1
-
 N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, 37°C, wild-type enzyme, addition of tissue factor Homo sapiens
3.4.21.21 1.2
-
 N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, 37°C, mutant enzyme M156Q, addition of tissue factor Homo sapiens
3.4.21.21 5.3
-
 N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, 37°C, mutant enzyme M156Q Homo sapiens
3.4.21.21 7.6
-
 N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 7.4, 37°C Homo sapiens
3.4.21.21 61
-
 Factor X pH 7.4, 37°C, wild-type enzyme Homo sapiens
3.4.21.21 68
-
 Factor X pH 7.4, 37°C, mutant enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.21 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.21 factor X + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.21 N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide + H2O i.e. chromozym t-PA Homo sapiens N-methylsulfonyl-D-Phe-Gly-Arg + p-nitroaniline
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.21 8
-
 Factor X pH 7.4, 37°C, wild-type enzyme Homo sapiens
3.4.21.21 10.2
-
 Factor X pH 7.4, 37°C, mutant enzyme Homo sapiens
3.4.21.21 12.1
-
 N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, 37°C, wild-type enzyme Homo sapiens
3.4.21.21 24.6
-
 N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, 37°C, mutant enzyme M156Q Homo sapiens
3.4.21.21 49.1
-
 N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, 37°C, wild-type enzyme, addition of tissue factor Homo sapiens
3.4.21.21 49.8
-
 N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide pH 8.0, 37°C, mutant enzyme M156Q, addition of tissue factor Homo sapiens